Genotyping and primer details

Genotyping and primer details for 588G12 - At3g47770
Line ID 588G12
Locus At3g47770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17618361 of line 588G12 Chr3:17618361
Insertion allele amplicon 8474 - 11yp
Locus specific primer 11yp: 5'-CATTGATATAACGAACATGCCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17617666
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11