Genotyping and primer details


Genotyping and primer details for 654A12 - At1g45688
Line ID 654A12
Locus At1g45688 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17192270 of line 654A12 Chr1:17192270
   
Insertion allele amplicon 8474 - LD22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LD22: 5'-GATTCTCTATGGTGCTGCTAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17191942
   
wt amplicon (over InsPos) AJ17 - LD22
wt amplicon start Chr1:17191942
wt amplicon end Chr1:17192890
wt amplicon size 949 bp
Locus specific primer LD22: 5'-GATTCTCTATGGTGCTGCTAAACC-3'
2nd locus specific primer AJ17: 5'-TTTTTCTTTTGTTCCTTCCTTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11