Genotyping and primer details


Genotyping and primer details for 655D01 - At2g40140
Line ID 655D01
Locus At2g40140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16773179 of line 655D01 Chr2:16773179
   
Insertion allele amplicon 8474 - 28nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28nt: 5'-GCAGAACCAGTAGAAGCATTAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16773510
   
wt amplicon (over InsPos) 06oe - 28nt
wt amplicon start Chr2:16772814
wt amplicon end Chr2:16773510
wt amplicon size 697 bp
Locus specific primer 28nt: 5'-GCAGAACCAGTAGAAGCATTAACC-3'
2nd locus specific primer 06oe: 5'-TGAATTACATAATTGCCACAGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11