Genotyping and primer details


Genotyping and primer details for 676A09 - At3g02830
Line ID 676A09
Locus At3g02830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:615454 of line 676A09 Chr3:615454
   
Insertion allele amplicon 8474 - 25dk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25dk: 5'-GAAGCTGTGTTGTCGTATGTGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:615792
   
Insertion allele amplicon 8474 - JV13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JV13: 5'-CTACCTCAGGGCAACAGTCTTATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:615038
   
wt amplicon (over InsPos) 25dk - JV13
wt amplicon start Chr3:615038
wt amplicon end Chr3:615792
wt amplicon size 755 bp
Locus specific primer JV13: 5'-CTACCTCAGGGCAACAGTCTTATC-3'
2nd locus specific primer 25dk: 5'-GAAGCTGTGTTGTCGTATGTGAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11