Genotyping and primer details


Genotyping and primer details for 684H02 - At5g45110
Line ID 684H02
Locus At5g45110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18230064 of line 684H02 Chr5:18230064
   
Insertion allele amplicon 8474 - CP35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP35: 5'-TTTCGAAAACTGAGAAACCAAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18229602
   
wt amplicon (over InsPos) 06bp - CP35
wt amplicon start Chr5:18229602
wt amplicon end Chr5:18230524
wt amplicon size 923 bp
Locus specific primer CP35: 5'-TTTCGAAAACTGAGAAACCAAAGT-3'
2nd locus specific primer 06bp: 5'-TCCTTTGGATTTGTCAGTCTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11