Genotyping and primer details


Genotyping and primer details for 701C12 - At4g01120
Line ID 701C12
Locus At4g01120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:482811 of line 701C12 Chr4:482811
   
Insertion allele amplicon 3144 - AJ15
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AJ15: 5'-AATTCAGCTGGAAACTCAGATCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:483164
   
Insertion allele amplicon 8474 - NE11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NE11: 5'-GATTCTCGGTTTGACTGTTTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:482394
   
wt amplicon (over InsPos) AJ15 - NE11
wt amplicon start Chr4:482394
wt amplicon end Chr4:483164
wt amplicon size 771 bp
Locus specific primer NE11: 5'-GATTCTCGGTTTGACTGTTTTCTC-3'
2nd locus specific primer AJ15: 5'-AATTCAGCTGGAAACTCAGATCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11