Genotyping and primer details


Genotyping and primer details for 710G03 - At1g49710
Line ID 710G03
Locus At1g49710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18392714 of line 710G03 Chr1:18392714
   
Insertion allele amplicon 8474 - NB92
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NB92: 5'-TTTTCTTGTTCATCAGGAGAGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18393009
   
wt amplicon (over InsPos) 11cr - NB92
wt amplicon start Chr1:18392212
wt amplicon end Chr1:18393009
wt amplicon size 798 bp
Locus specific primer NB92: 5'-TTTTCTTGTTCATCAGGAGAGGTT-3'
2nd locus specific primer 11cr: 5'-AAGCATGAAGCTTTCAAGAAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11