Genotyping and primer details


Genotyping and primer details for 726G07 - At1g07000
Line ID 726G07
Locus At1g07000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2151609 of line 726G07 Chr1:2151609
   
Insertion allele amplicon 8474 - DN39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DN39: 5'-ATCCCTCTAATCGCTTCTCCTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2151119
   
wt amplicon (over InsPos) 10dk - DN39
wt amplicon start Chr1:2151119
wt amplicon end Chr1:2152216
wt amplicon size 1098 bp
Locus specific primer DN39: 5'-ATCCCTCTAATCGCTTCTCCTAAC-3'
2nd locus specific primer 10dk: 5'-CTTACAGATTCTCTCCGGTTATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11