Genotyping and primer details

Genotyping and primer details for 770C01 - At5g12370
Line ID 770C01
Locus At5g12370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4005603 of line 770C01 Chr5:4005603
Insertion allele amplicon 8474 - 15um
Locus specific primer 15um: 5'-TTTTGTTTTGTCATGCAAACTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4005930
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11