Genotyping and primer details


Genotyping and primer details for 797B09 - At4g20140
Line ID 797B09
Locus At4g20140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10886369 of line 797B09 Chr4:10886369
   
Insertion allele amplicon 8474 - EU70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU70: 5'-GAACCTCACCAGAATCAATCTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10885875
   
wt amplicon (over InsPos) BB08 - EU70
wt amplicon start Chr4:10885875
wt amplicon end Chr4:10886600
wt amplicon size 726 bp
Locus specific primer EU70: 5'-GAACCTCACCAGAATCAATCTCTC-3'
2nd locus specific primer BB08: 5'-AGCTTTGATAATGTCCCAATTGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11