Genotyping and primer details


Genotyping and primer details for 833C07 - At1g51600
Line ID 833C07
Locus At1g51600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19134883 of line 833C07 Chr1:19134883
   
Insertion allele amplicon 8474 - IF40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IF40: 5'-TATGTCATTGGCCACTGTTATCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19135237
   
wt amplicon (over InsPos) 36hs - IF40
wt amplicon start Chr1:19134238
wt amplicon end Chr1:19135237
wt amplicon size 1000 bp
Locus specific primer IF40: 5'-TATGTCATTGGCCACTGTTATCAT-3'
2nd locus specific primer 36hs: 5'-ACTGCGAGTTCCTGACATACTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11