Genotyping and primer details


Genotyping and primer details for 845F03 - At5g59670
Line ID 845F03
Locus At5g59670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24042987 of line 845F03 Chr5:24042987
   
Insertion allele amplicon 8474 - EU80
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU80: 5'-AATGGAATTCAGAAAAATTGGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24042704
   
wt amplicon (over InsPos) BB15 - EU80
wt amplicon start Chr5:24042704
wt amplicon end Chr5:24043636
wt amplicon size 933 bp
Locus specific primer EU80: 5'-AATGGAATTCAGAAAAATTGGATG-3'
2nd locus specific primer BB15: 5'-ACTGAGTTGGAATCTGAAAAGCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11