Genotyping and primer details


Genotyping and primer details for 857H06 - At3g02130
Line ID 857H06
Locus At3g02130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:382131 of line 857H06 Chr3:382131
   
Insertion allele amplicon 8474 - BB87
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BB87: 5'-ATATGTCAAAGCTGCCATCTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:382714
   
Insertion allele amplicon 8474 - EU84
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU84: 5'-AGCTTGAGGTTTTGGATGTTTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:381657
   
wt amplicon (over InsPos) BB87 - EU84
wt amplicon start Chr3:381657
wt amplicon end Chr3:382714
wt amplicon size 1058 bp
Locus specific primer EU84: 5'-AGCTTGAGGTTTTGGATGTTTCTA-3'
2nd locus specific primer BB87: 5'-ATATGTCAAAGCTGCCATCTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11