Genotyping and primer details


Genotyping and primer details for 859H07 - At2g29680
Line ID 859H07
Locus At2g29680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12690784 of line 859H07 Chr2:12690784
   
Insertion allele amplicon 8474 - HV52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HV52: 5'-ACCAATCTTTGTGCATGTTTCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12690299
   
wt amplicon (over InsPos) 09dk - HV52
wt amplicon start Chr2:12690299
wt amplicon end Chr2:12691270
wt amplicon size 972 bp
Locus specific primer HV52: 5'-ACCAATCTTTGTGCATGTTTCATA-3'
2nd locus specific primer 09dk: 5'-TTCCTTGAATACCATTAATCAGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11