Genotyping and primer details


Genotyping and primer details for SALK_002232c - At1g71190
Line ID SALK_002232c
Locus At1g71190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26833787 of line SALK_002232c Chr1:26833787
   
Insertion allele amplicon LBb3 - 02rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02rb: 5'-GGCTATTTTATTGCCTCCAATGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26834100
   
wt amplicon (over InsPos) 27rz - 02rb
wt amplicon start Chr1:26833381
wt amplicon end Chr1:26834100
wt amplicon size 720 bp
Locus specific primer 02rb: 5'-GGCTATTTTATTGCCTCCAATGTA-3'
2nd locus specific primer 27rz: 5'-AAGAACATCGAGTCTCACCAATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11