Genotyping and primer details


Genotyping and primer details for SALK_004447 - At1g17750
Line ID SALK_004447
Locus At1g17750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6108631 of line SALK_004447 Chr1:6108631
   
Insertion allele amplicon LBb3 - 50cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50cs: 5'-TTCATCTTGGGTTCTAATCAGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6107976
   
wt amplicon (over InsPos) 24dw - 50cs
wt amplicon start Chr1:6107976
wt amplicon end Chr1:6108897
wt amplicon size 922 bp
Locus specific primer 50cs: 5'-TTCATCTTGGGTTCTAATCAGCTT-3'
2nd locus specific primer 24dw: 5'-ATAGTGAGGACCCAGCTGCTATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11