Genotyping and primer details


Genotyping and primer details for SALK_007247c - At1g20810
Line ID SALK_007247c
Locus At1g20810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7233332 of line SALK_007247c Chr1:7233332
   
Insertion allele amplicon R204 - BL09
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BL09: 5'-GGTTTTGAGAGTTTCTGATTCGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7232405
   
Insertion allele amplicon LBb3 - BL10
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BL10: 5'-CTTTACACAAATGTTGGGACAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7233421
   
wt amplicon (over InsPos) BL10 - BL09
wt amplicon start Chr1:7232405
wt amplicon end Chr1:7233421
wt amplicon size 1017 bp
Locus specific primer BL09: 5'-GGTTTTGAGAGTTTCTGATTCGAC-3'
2nd locus specific primer BL10: 5'-CTTTACACAAATGTTGGGACAATG-3'
   
wt amplicon (over InsPos) 04mg - 03mg
wt amplicon start Chr1:7232851
wt amplicon end Chr1:7233929
wt amplicon size 1079 bp
Locus specific primer 03mg: 5'-GTTTGATGGTGGCTTACTTCTTTC-3'
2nd locus specific primer 04mg: 5'-GCCAATAATTTGAAGAAATTCACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11