Sequences from confirmation amplicon


Confirmation sequences for line SALK_007247c and hit At1g20810 are shown.
Line ID SALK_007247c
Locus At1g20810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7233332
Primers used for PCR BL09 - R204, show primer details
   
Sequence Name 67-K105636-SALK_007247C-BL09
GenBank Accession LN485916 [GenBank]
Sequencing primer gene specific primer, BL09: 5'-GGTTTTGAGAGTTTCTGATTCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K105636-SALK_007247C-BL09
GATTTGCTTGATTGTATGAGAAGTTTCTGGGTTTGTAAAACATAAACTTTAGCTTGTTAT
TAACACAATGTTTGTGTTACTCTGTTCTTGCAGCTGAAGGGTTGAAATTCTATGACATTG
AGGAAGGCAAAGGTCCAGTAGCAACAGAGGGATCAACTGCTCAGGTCTCTCTAGCTAATG
ATTCACATTTTAATTTCTCTATATCGAAATCGAGTTCTCGCTTTTACAATGTAATGGTGT
CTGTATTTTCCACATTTAGGTGCATTTTGATTGCCGTTACAGAAGCATCACTGCAATTTC
TACCCGAGAATCCAAGCTTTTAGCTGGAAACCGTAGTATTGCTCAGGTTACTTCACCATC
TATCCCTTTTGATTAGATTCTGCATAATTTTTAGTTTGATGGTGGCTTACTTCTTTCTGG
AGCAGCCTTACGAGTTCAAGGTGGGATCTACGCCAGGAAAGGAAAGGAAGCGTGAATTCG
TTGATAATCCAAATGGGTTATTCTCTGCACAGGCTGCACCAAAACCTCCTCCAGCAATGT
ATTTCATTACCGAAGGAATGAAAGTCGGAGGCAAGGTTAGAAACATTACTGCATTTTCGC
TCTCTTCTTCTTACAACATGTAGTTACATATAAGTAGTTTTGACCTATGTTGTTCTTGCA
GAGAACGGTGATTGTTCCTCCTGAAGCTGGTTATGGTCAGAAAGGAATGAATGAGATACC
GGTGAGAACCTACTACAAATTGTATATGAGGAAAGCTCCGTTTTTTGCTGAAAACTAATT
ACAAAATGTAGTATAATGCTTGTTTCGGGTTTAAGAGATAATAAAATCATGTATGTTGTT
GCAGCCTGGAGCTACTTTTGAGTTAAACATAGAGCAACAAATTGACGTATTAGACAAATT
GACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTATGTACTGGGGTGGTTTTCTT
TTCCCAGTGAGACGGGCACAGCTGATTGCCTTCACGCCTGGCCCTG
   
Sequence Name 71-K105637-SALK_007247C-R210
GenBank Accession LN486020 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K105637-SALK_007247C-R210
GTCATTTGTCAATACGTCAATTTGTTGCTCTATGTTTAACTCAAAAGTAGCTCCAGGCTG
CAACAACATACATGATTTTATTATCTCTTAAACCCGAAACAAGCATTATACTACATTTTG
TAATTAGTTTTCAGCAAAAAACGGAGCTTTCCTCATATACAATTTGTAGTAGGTTCTCAC
CGGTATCTCATTCATTCCTTTCTGACCATAACCAGCTTCAGGAGGAACAATCACCGTTCT
CTGCAAGAACAACATAGGTCAAAACTACTTATATGTAACTACATGTTGTAAGAAGAAGAG
AGCGAAAATGCAGTAATGTTTCTAACCTTGCCTCCGACTTTCATTCCTTCGGTAATGAAA
TACATTGCTGGAGGAGGTTTTGGTGCAGCCTGTGCAGAGAATAACCCATTTGGATTATCA
ACGAATTCACGCTTCCTTTCCTTTCCTGGCGTAGATCCCACCTTGAACTCGTAAGGCTGC
TCCAGAAAGAAGTAAGCCACCATCAAACTAAAAATTATGCAGAATCTAATCAAAAGGGAT
AGATGGTGAAGTAACCTGAGCAATACTACGGTTTCCAGCTAAAAGCTTGGATTCTCGGGT
AGAAATTGCAGTGATGCTTCTGTAACGGCAATCAAAATGCACCTAAATGTGGAAAATACA
GACACCATTACATTGTAAAAGCGAGAACTCGATTTCGATATAGAGAAATTAAAATGTGAA
TCATTAGCTAGAGAGACCTGAGCAGTTGATCCCTCTGTTGCTACTGGACCTTTGCCTTCC
TCAATGTCATAGAATTTCAACCCTTCAGCTGCAAGAACAGAGTAACACAAACATTGTGTT
AATAACAAGCTAAAGTTTATGTTTTTACAAACCCAGAAACTTCTCATACAAGTCAAGCAA
ATCCTTTTATGTCCCCCAATTCAGAATTTCGTCG
Primers used for PCR BL10 - LBb3, show primer details
   
Sequence Name 73-K119131-SALK_007247C-BL10
GenBank Accession LN486094 [GenBank]
Sequencing primer gene specific primer, BL10: 5'-CTTTACACAAATGTTGGGACAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K119131-SALK_007247C-BL10
ACTCAAAAGTATACTCCAGGCTGCAACAACATACATGATTTTATTATCTCTTAAACCCGA
AACAAGCATTATACTACATTTTGTAATTAGTTTTCAGCAAAAAACGGAGCTTTCCTCATA
TACAATTTGTAGTAGGTTCTCACCGGTATCTCATTCATTCCTTTCTGACCATAACCAGCT
TCAGGAGGAACAATCACCGTTCTCTGCAAGAACAACATAGGTCAAAACTACTTATATGTA
ACTACATGTTGTAAGAAGAAGAGAGCGAAAATGCAGTAATGTTTCTAACCTTGCCTCCGA
CTTTCATTCCTTCGGTAATGAAATACATTGCTGGAGGAGGTTTTGGTGCAGCCTGTGCAG
AGAATAACCCATTTGGATTATCAACGAATTCACGCTTCCTTTCCTTTCCTGGCGTAGATC
CCACCTTGAACTCGTAAGGCTGCTCCAGAAAGAAGTAAGCCACCATCAAACTAAAAATTA
TGCAGAATCTAATCAAAAGGGATAGATGGTGAAGTAACCTGAGCAATACTACGGTTTCCA
GCTAAAAGCTTGGATTCTCGGGTAGAAATTGCAGTGATGCTTCTGTAACGGCAATCAAAA
TGCACCTAAATGTGGAAAATACAGACACCATTACATTGTAAAAGCGAGAACTCGATTTCG
ATATAGAGAAATTAAAATGTGAATCATTAGCTAGAGAGACCTGAGCAGTTGATCCCTCTG
TTGCTACTGGACCTTTGCCTTCCTCAATGTCATAGAAATTCAACCTTCAGCTGCAAGAAC
AGAGTAACACAAACATTGTG
   
Sequence Name 59-K119130-SALK_007247C-R210
GenBank Accession LN485727 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K119130-SALK_007247C-R210
AACTCAATGATCCATCTGTTTTCCAAGATATCTTTCCCGTTTTTCTTGAACAAGCTCTGA
TCATATGGTGAGATTCCACTAGTCAATCTCATAACTTTGCTTATAGAGGAGACACGTTTA
TCATCATCATCATCATCGTTAACCATCGTCATCATGTCGACGTCCTCCTTCTCATCTAGT
TCCATCGAAGAGGATGCCGTCAAAGACGGTTTGGACTGACACAGGTCTCGTTTACCATCG
TACCACCGTGGAATCCAACCCATGACTCCCCACTTGGTAGCTTTAAAGTCATATCCATGA
ATCACTCCGTCGTCGTTACAGTCCTTCTTACATACAGTTTTGAAATAAGGATCGTCGGAT
ATATGCGAGTTGATAGATCGATCCGTGCATTGCATCATTGCCAGAGATTTTGGATCTAAT
TTGAAGAGAATCTCTTCAAAGATATGAAAAGGTAATCCATCCATGATTGATCTAATTAAT
AAGAAAAAGAGACAAAACAAGATTAGGGCTTTTTGCTCCGTTCCGTGATGGTGACTATTC
CAGAAGTTTTGGTGATGTTATAGTAGATATGATGTATTTATAGACGTAGATTATTTGCGC
TTTTACATATTAAAAAGGAAAGATTT

Last Updated on 06.12.2018 16:11