Genotyping and primer details


Genotyping and primer details for SALK_009022 - At1g14580
Line ID SALK_009022
Locus At1g14580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4989899 of line SALK_009022 Chr1:4989899
   
Insertion allele amplicon LBb3 - 80sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 80sm: 5'-CAAGAGAGAATTGTATGCAAGCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4990444
   
wt amplicon (over InsPos) 05tp - 80sm
wt amplicon start Chr1:4989420
wt amplicon end Chr1:4990444
wt amplicon size 1025 bp
Locus specific primer 80sm: 5'-CAAGAGAGAATTGTATGCAAGCAC-3'
2nd locus specific primer 05tp: 5'-GGTGAATCTCACATTAAATCCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11