Genotyping and primer details


Genotyping and primer details for SALK_009935c - At3g15940
Line ID SALK_009935c
Locus At3g15940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5394483 of line SALK_009935c Chr3:5394483
   
Insertion allele amplicon LBb3 - 07ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07ii: 5'-CAATATTCCTTTCTGCCTCAAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5395026
   
wt amplicon (over InsPos) 08ir - 07ii
wt amplicon start Chr3:5394198
wt amplicon end Chr3:5395026
wt amplicon size 829 bp
Locus specific primer 07ii: 5'-CAATATTCCTTTCTGCCTCAAGTT-3'
2nd locus specific primer 08ir: 5'-ACAGAACCGAGTTCGATAAGTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11