Genotyping and primer details


Genotyping and primer details for SALK_011936c - At3g57940
Line ID SALK_011936c
Locus At3g57940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21451976 of line SALK_011936c Chr3:21451976
   
Insertion allele amplicon LBb3 - 03gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03gq: 5'-TATTTCATTGAGAAGAACCTGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21451359
   
wt amplicon (over InsPos) 21hs - 03gq
wt amplicon start Chr3:21451359
wt amplicon end Chr3:21452130
wt amplicon size 772 bp
Locus specific primer 03gq: 5'-TATTTCATTGAGAAGAACCTGCAA-3'
2nd locus specific primer 21hs: 5'-GGATGATAGAGCGGTCTCTAAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11