Genotyping and primer details


Genotyping and primer details for SALK_012172c - At4g31570
Line ID SALK_012172c
Locus At4g31570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15306410 of line SALK_012172c Chr4:15306410
   
Insertion allele amplicon R212 - 03qm
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 03qm: 5'-TGCAAAGAGTAGCTTGATTTGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15306097
   
Insertion allele amplicon LBb3 - 11pt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11pt: 5'-TTGATTGGATTGATTCTTACCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15307091
   
wt amplicon (over InsPos) 03qm - 11pt
wt amplicon start Chr4:15306097
wt amplicon end Chr4:15307091
wt amplicon size 995 bp
Locus specific primer 11pt: 5'-TTGATTGGATTGATTCTTACCTCC-3'
2nd locus specific primer 03qm: 5'-TGCAAAGAGTAGCTTGATTTGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11