Sequences from confirmation amplicon


Confirmation sequences for line SALK_012172c and hit At4g31570 are shown.
Line ID SALK_012172c
Locus At4g31570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15306410
Primers used for PCR 11pt - LBb3, show primer details
   
Sequence Name 43-K115373-SALK_012172C-11pt
GenBank Accession LN485356 [GenBank]
Sequencing primer gene specific primer, 11pt: 5'-TTGATTGGATTGATTCTTACCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115373-SALK_012172C-11pt
TCAGTTCACGCTCAGTAACTCGCGGGTCCAATTTCCGACGTACACACGTGACATTACTTC
TGTAACCTGTCAATGGCACAATAAACCAAGGTCAACATAAAGGAATTCTAAATCTTTTCT
AGTAAGACATTCGAAAGGATTTGAATTCATCGAGAAAAAATACAGAGTCACTAGATCCAC
CAAGTGACCCGATCAAATTTGAACAGATCGGATCAACGTTACCACAATTAAGAGTGAGAA
GGAGATAAAGAAACTTGGTTGCGACCTACCTTGTGGGAAGAGCTGAAAGCACTCAAGGGG
CAAAAAGAATGGCTTTTTCTGTGTTTTGCTTTGTGAAGAAATTAGGGTTTCTGAATCTGA
AATTTGATGAAATACTTTCCAAAGGGTTTTGTAAGCGCGTTAACAAAATTTAGTAAATAT
ACAATTTGGGCCTATTTATTGGGCTTAAAAGTAGCCTTTTCATGCCTTTTTTCAATAATA
TGTTAGTGTCGGTGATATTTATTTTTTCTCTGTATAGTTTGTTAAATACTTAAATGTGAT
CATTTCACGTATTTTGCTTTGTATTTCTGAAAATAATGAATAGCAGAATAGGGAAAAAGA
AAAATAATAGAACTTCAAGTCTCTCTACAACTGACGCTTATTGTGGTGTAAACAAATTGA
CGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
   
Sequence Name 41-K115372-SALK_012172C-R210
GenBank Accession LN485314 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115372-SALK_012172C-R210
CATTTGTTTACCCACAATAAGCGTCAGTTGTAGAGAGACTTGAAGTTCTATTATTTTTCT
TTTTCCCTATTCTGCTATTCATTATTTTCAGAAATACAAAGCAAAATACGTGAAATGATC
ACATTTAAGTATTTAACAAACTATACAGAGAAAAAATAAATATCACCGACACTAACATAT
TATTGAAAAAAGGCATGAAAAGGCTACTTTTAAGCCCAATAAATAGGCCCAAATTGTATA
TTTACTAAATTTTGTTAACGCGCTTACAAAACCCTTTGGAAAGTATTTCATCAAATTTCA
GATTCAGAAACCCTAATTTCTTCACAAAGCAAAACACAGAAAAAGCCATTCTTTTTGCCC
CTTGAGTGCTTTCAGCTCTTCCCACAAGGTAGGTCGCAACCAAGTTTCTTTATCTCCTTC
TCACTCTTAATTGTGGTAACGTTGATCCGATCTGTTCAAATTTGATCGGGTCACTTGGTG
GATCTAGTGACTCTGTATTTTTTCTCGATGAATTCAAATCCTTTCGAATGTCTTACTAGA
AAAGATTTAGAATTCCTTTATGTTGACCTTGGTTTATTGTGCCATTGACAGGTTACAGAA
GTAATGTCACGTGTGTACGTCGGAAATTTGGACCCGCGAGTTACTGAGCGTGAACTTGAG
GATGAGTTCCGTGCCTTTGGAGTTGTCAGGAGGTAAG
Primers used for PCR 03qm - R212, show primer details
   
Sequence Name 62-K118240-SALK_012172C-03qm
GenBank Accession LN485806 [GenBank]
Sequencing primer gene specific primer, 03qm: 5'-TGCAAAGAGTAGCTTGATTTGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118240-SALK_012172C-03qm
GCATGCATGGGCTCATCAAAGTGGTGATGTGTTGTCGTCTCCAACACTAATTTTTTTTTT
TTTTTTTTTTTTTTTTTTGCCCCCCCCCCTTTTTTAAAAACCCGGGGGAACCAAAAAAAA
CCCCCACAAAGGGGGGGCCCCCCCTTTTTAAGGGCCCAAAACAAAAACGGGGGGGGTTTC
CAAAAAAAAAAAAAAAAAATTTTTTTTTAACCCGGGGGAAAAAAAAAAAACCAAATGGGA
AAAAAAAAAAGCCCCCCAAATTTTAAAAAAGGGGGGGGGAAAAAAACACCTAAAAAAAGG
GGAAAAAAAAGGGGGGCCCCTTTAACCCCCCCCCCAAAAACCGGGAAAAACCCTTTTTTT
TTGGGAAAAAAAAAAACCCCCCCCCTAAAAAGCCCCCCCCCCCCCGGGGTTTTGGGGTTT
AAAAGGACAAACCCCACCCCCAAAAAACCTTTTGGGGCCCCAAAAAAAAACCAAAAGG
   
Sequence Name 39-K118239-SALK_012172C-R214
GenBank Accession LN485287 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K118239-SALK_012172C-R214
CTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTATCA
GTGTCAATCTCTACTTCACAATTTGTATATCTTTCATTCCACAGCTACTAAATAAACTTT
CTTTCTTATTCGTTTGAAAACCCAAGCAGTTTGTGATTCGGATCAATAAAAATGCTGAAA
CCACAGTTTGCTTGGTTAATATAAGCTAAACAATGAATTAAAAACAAAGACAAAGACAAA
AAAAAAAAAAAAAAAAAAAAAAAATTTTTGGGGGGGAAAAAAACCCCCCCCCCCTTTTTA
AAACCCCCCGGGGTTTTAAAAAAAAAACCCCCCCAAAAATTAAAAAAAAAAACCCTTTTA
AAAATTTAAAAAAAACCCCTTTTTTTTTAAAA

Last Updated on 06.12.2018 16:11