Genotyping and primer details


Genotyping and primer details for SALK_012890c - At1g63930
Line ID SALK_012890c
Locus At1g63930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23728157 of line SALK_012890c Chr1:23728157
   
Insertion allele amplicon LBb3 - 21ih
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21ih: 5'-ATTACGAAAACCATCACCGTACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23728747
   
wt amplicon (over InsPos) 10kj - 21ih
wt amplicon start Chr1:23727749
wt amplicon end Chr1:23728747
wt amplicon size 999 bp
Locus specific primer 21ih: 5'-ATTACGAAAACCATCACCGTACTC-3'
2nd locus specific primer 10kj: 5'-ACCTCTACAAGGACAAAACTGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11