Sequences from confirmation amplicon


Confirmation sequences for line SALK_012890c and hit At1g63930 are shown.
Line ID SALK_012890c
Locus At1g63930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23728157
Primers used for PCR 21ih - LBb3, show primer details
   
Sequence Name 13-K113191-SALK_012890C-21ih
GenBank Accession LN484643 [GenBank]
Sequencing primer gene specific primer, 21ih: 5'-ATTACGAAAACCATCACCGTACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K113191-SALK_012890C-21ih
CATCCCGCCGCTTCGTTACCCGTGGCGGCGTAAGATTCGCTGTCATTGCGTGGATCTGCT
TCGCCGCCGACCAATTTCTCCCAACCGCCCACGACGACGATTTGAGCTGACCAATCGTTG
CTCCTCCTTTACTCGCCGCCGATGATCCACCGCTTCGACGACCAAACGACCACGATCTCT
CCGTCGTCTTATTCCCTCCGCCGCCGCCACCACCTCCGCCGCTAACATTCTCTTTATCCT
CGAGACTCAACGCGACGACGAGATTCGCCAACGCGCGTTTAGCTCTCCTCACGTTACCGT
CACCTAACGGACGTTGCTCCAAAGCCGTCACAGCAATCTCAGCTAAACGCTGGTAATGTC
TAACGGAATCGATACCGTTAACAACGGCGGTACAAATGTCAAGCGCTTTAATCGACCGGT
CTAACATTTCAGGAACTAACCGGTCAAAAGGCGGCTTAGAAATCTGGGTCGGGTCACGAC
CCATTAACAAAATCGCTTTGAATTCAGCTTCGCAACAGAGGAAAACATCCATCAGCTTAC
GAAGCGGTCGCGGACAAATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATA
ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC
AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG
GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCCG
   
Sequence Name 02-K113190-SALK_012890C-R210
GenBank Accession LN484309 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K113190-SALK_012890C-R210
ATATATTTGTCCGCGACCGCTTCGTAAGCTGATGGATGTTTTCCTCTGTTGCGAAGCTGA
ATTCAAAGCGATTTTGTTAATGGGTCGTGACCCGACCCAGATTTCTAAGCCGCCTTTTGA
CCGGTTAGTTCCTGAAATGTTAGACCGGTCGATTAAAGCGCTTGACATTTGTACCGCCGT
TGTTAACGGTATCGATTCCGTTAGACATTACCAGCGTTTAGCTGAGATTGCTGTGACGGC
TTTGGAGCAACGTCCGTTAGGTGACGGTAACGTGAGGAGAGCTAAACGCGCGTTGGCGAA
TCTCGTCGTCGCGTTGAGTCTCGAGGATAAAGAGAATGTTAGCGGCGGAGGTGGTGGCGG
CGGCGGAGGGAATAAGACGACGGAGAGATCGTGGTCGTTTGGTCGTCGAAGCGGTGGATC
ATCGGCGGCGAGTAAAGGAGGAGCAACGATTGGTCAGCTCAAATCGTCGTCGTGGGCGGT
TGGGAGAAATTGGTCGGCGGCGAAGCAGATCCACGCAATGACAGCGAATCTTACGCCGCC
ACGTGGTAACGAAGCGGCGGGATTGCCTCAACCAATGTTTATAATGAGTACGGTGATGGT
TTTCGTAAT

Last Updated on 06.12.2018 16:11