Genotyping and primer details


Genotyping and primer details for SALK_014538 - At1g73080
Line ID SALK_014538
Locus At1g73080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27484853 of line SALK_014538 Chr1:27484853
   
Insertion allele amplicon LBb3 - 17ce
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17ce: 5'-CAGAGTAGTGAGATTCCCCAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27485256
   
wt amplicon (over InsPos) 18dn - 17ce
wt amplicon start Chr1:27484590
wt amplicon end Chr1:27485256
wt amplicon size 667 bp
Locus specific primer 17ce: 5'-CAGAGTAGTGAGATTCCCCAAAAG-3'
2nd locus specific primer 18dn: 5'-TTCTTGTTTAAACTCAGATGGGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11