Genotyping and primer details


Genotyping and primer details for SALK_014827c - At3g13980
Line ID SALK_014827c
Locus At3g13980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4619283 of line SALK_014827c Chr3:4619283
   
Insertion allele amplicon LBb3 - 32dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32dh: 5'-TCGAGAGATCTAGGAGGAAGACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4620036
   
wt amplicon (over InsPos) 86dy - 32dh
wt amplicon start Chr3:4618856
wt amplicon end Chr3:4620036
wt amplicon size 1181 bp
Locus specific primer 32dh: 5'-TCGAGAGATCTAGGAGGAAGACAG-3'
2nd locus specific primer 86dy: 5'-AAAGAAAAGACAACGACTCTCTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11