Sequences from confirmation amplicon


Confirmation sequences for line SALK_014827c and hit At3g13980 are shown.
Line ID SALK_014827c
Locus At3g13980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4619283
Primers used for PCR 32dh - LBb3, show primer details
   
Sequence Name 23-K109819-SALK_014827C-32dh
GenBank Accession LN484943 [GenBank]
Sequencing primer gene specific primer, 32dh: 5'-TCGAGAGATCTAGGAGGAAGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K109819-SALK_014827C-32dh
TCACTCTGCTCTTCTGATTCAGCGGATTCTCCTCTTCCGAGTCAGATTCATTCCACGGAC
GCTCCAAATCTTCCGCTTCTCCTCCTTCTTCTTCTCGTCAACAACCGAAACCGATTCGAA
CATCATCCGTCGACCACTCCTCCGCCGTCCAGAAACCTAAAGAACTCGGTGGCTTCTTGA
GAACGAAATCAAAAGCGTTAAAGATCTACTCCGACTTGAAAAAAGTCAAACAACCTATCT
CTCCGGGCGGCAGACTCGCTACTTTCCTTAACTCTCTTTTCACCAACGCCGCCACTAACC
CTAAGAAGCACAAGAAAACTACCACCGTCGCCGTCGTGGAAGAACCACACTCCTCCTCCA
CATGCTCCTCCGCGTCGTCCTTCTCTAGGTCTTGCTTGAGCAAAACTCCATCGTCTAGCG
GCAAATCAAAAAGATCCGTACGGTTCTGTCCAGTCAATGTGATCCTTGACGAAGATTCCT
CCTTTACCATGCCTTACGCTTATAACAACGAAAGACTCTACGATAACAACGAAGCTNAAC
GCGTGGAAGAGCATCGCCGTGTCATCCAAGCAGCCAAAGATCTTCTTAGAACTTACCATA
ACAAGAACAAAGTTACAACTACGAACATCAATAACGTAGAAGAAGATGATGAAGACGATG
CTGCGAGTTGTGCAAGCTCTGATCTATTCGAGTTGGAGAATCTGTCGGCTATTCGAGTTG
ACGCTTAGACNACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCCGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCC
   
Sequence Name 07-K109818-SALK_014827C-R210
GenBank Accession LN484455 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K109818-SALK_014827C-R210
ACTCGATAGCCGACAGATTCTCCACTCGAATAGATCAGAGCTTGCACAACTCGCAGCATC
GTCTTCATCATCTTCTTCTACGTTATTGATGTTCGTAGTTGTAACTTTGTTCTTGTTATG
GTAAGTTCTAAGAAGATCTTTGGCTGCTTGGATGACACGGCGATGCTCTTCCACGCGTTT
AGCTTCGTTGTTATCGTAGAGTCTTTCGTTGTTATAAGCGTAAGGCATGGTAAAGGAGGA
ATCTTCGTCAAGGATCACATTGACTGGACAGAACCGTACGGATCTTTTTGATTTGCCGCT
AGACGATGGAGTTTTGCTCAAGCAAGACCTAGAGAAGGACGACGCGGAGGAGCATGTGGA
GGAGGAGTGTGGTTCTTCCACGACGGCGACGGTGGTAGTTTTCTTGTGCTTCTTAGGGTT
AGTGGCGGCGTTGGTGAAAAGAGAGTTAAGGAAAGTAGCGAGTCTGCCGCCCGGAGAGAT
AGGTTGTTTGACTTTTTTCAAGTCGGAGTAGATCTTTAACGCTTTTGATTTCGTTCTCAA
GAAGCCACCGAGTTCTTTAGGTTTCTGGACGGCGGAGGAGTGGTCGACGGATGATGTTCG
AATCGGTTTCGGTTGTTGACGAGAAGAAGAAGGAGGAGAAGCGGAAGATTTGGAGCGTCC
GTGGAATGAATCTGACTCGGAAGAGGAGAATCCGCTTGAATCAGAAGAGCTAGAGTTGGA
ATGTCTGAGAAAGTCTGTCTTCCTCCT

Last Updated on 06.12.2018 16:11