Genotyping and primer details


Genotyping and primer details for SALK_015044c - At1g80310
Line ID SALK_015044c
Locus At1g80310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30195975 of line SALK_015044c Chr1:30195975
   
Insertion allele amplicon LBb3 - 22ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22ux: 5'-GCCATCAAATACGTCAGGTTTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30195401
   
wt amplicon (over InsPos) 52vl - 22ux
wt amplicon start Chr1:30195401
wt amplicon end Chr1:30196519
wt amplicon size 1119 bp
Locus specific primer 22ux: 5'-GCCATCAAATACGTCAGGTTTAAT-3'
2nd locus specific primer 52vl: 5'-CACAAAGTTGAGCAATGAAAACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11