Sequences from confirmation amplicon


Confirmation sequences for line SALK_015044c and hit At1g80310 are shown.
Line ID SALK_015044c
Locus At1g80310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30195975
Primers used for PCR 22ux - LBb3, show primer details
   
Sequence Name 18-K117668-SALK_015044C-R210
GenBank Accession LN484794 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117668-SALK_015044C-R210
TCATTTGTTTACCAAACCGATACTGACCAGCTAACCCACCAGCACCGTGACAGACGGGCA
TAGCGCCAAACCAGCACCCTATTAAGTTCATCACCCCAACGCTGATGGAGACTGTAGTCG
CAGAGAGTTCCTTGTCAAACAAGTCATTGGATAATTTACAAACTGCGATCACTGAGTTCA
GTACAGAGAGTGGAATCTGAGGAATCGCCGCCCTCAGAAACCCGATTTTCCAATCATCCC
AACTGATTCTCAGAATGTGGAACTTCGAGGGACCGAATTTAAGGTCTTTGAAAATGGATG
GATCACGTATGAAACAGAGCACTAACCCGAGTGCGAACACGATCAGCGCAGATGGAATCG
AAGACAGAAGACGCAGTCTCCTCCGGCGAGACTGGCTTTCGTTGCTGGAAGTCTCGGCGA
GATCTCCATCTTCAGCTTCTCTGTCGTTGCCAGAGCCGGTGGACAAAATGATGAAGAGCA
GAGCAGCTAGAGCCAAGATAAGGCCGTCGAGGCCAAGCCAAATACGAGGAGAAGAAGAGG
GTTTGAGAGTGGCAGTATCGTAATTAAACCTGACGTA
   
Sequence Name 55-K117669-SALK_015044C-22ux
GenBank Accession LN485629 [GenBank]
Sequencing primer gene specific primer, 22ux: 5'-GCCATCAAATACGTCAGGTTTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K117669-SALK_015044C-22ux
CTCTTCTTTCCTCGTATTTGGCTTGGCCTCGACGGCCTTATCTTGGCTCTAGCTGCTCTG
CTCTTCATCATTTTGTCCACCGGCTCTGGCAACGACAGAGAAGCTGAAGATGGAGATCTC
GCCGAGACTTCCAGCAACGAAAGCCAGTCTCGCCGGAGGAGACTGCGTCTTCTGTCTTCG
ATTCCATCTGCGCTGATCGTGTTCGCACTCGGGTTAGTGCTCTGTTTCATACGTGATCCA
TCCATTTTCAAAGACCTTAAATTCGGTCCCTCGAAGTTCCACATTCTGAGAATCAGTTGG
GATGATTGGAAAATCGGGTTTCTGAGGGCGGCGATTCCTCAGATTCCACTCTCTGTACTG
AACTCAGTGATCGCAGTTTGTAAATTATCCAATGACTTGTTTGACAAGGAACTCTCTGCG
ACTACAGTCTCCATCAGCGTTGGGGTGATGAACTTAATAGGGTGCTGGTTTGGCGCTATG
CCCGTCTGTCACGGTGCTGGTGGGTTAGCTGGTCAGTATCGGTTTGGTGTAAACAAATTG
ACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
G

Last Updated on 06.12.2018 16:11