Genotyping and primer details


Genotyping and primer details for SALK_015182c - At5g61590
Line ID SALK_015182c
Locus At5g61590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24765075 of line SALK_015182c Chr5:24765075
   
Insertion allele amplicon LBb3 - 45xl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45xl: 5'-CCTAGCAAGACAACCCTACAAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24766008
   
Insertion allele amplicon R204 - AD08
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AD08: 5'-CTCTGAATAAACCGAAAGGACAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24764431
   
wt amplicon (over InsPos) 09au - AD08
wt amplicon start Chr5:24764431
wt amplicon end Chr5:24765138
wt amplicon size 708 bp
Locus specific primer AD08: 5'-CTCTGAATAAACCGAAAGGACAAG-3'
2nd locus specific primer 09au: 5'-GGACTCTGGTCTCTAGAACCCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11