Genotyping and primer details


Genotyping and primer details for SALK_017267 - At2g45440
Line ID SALK_017267
Locus At2g45440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18732458 of line SALK_017267 Chr2:18732458
   
Insertion allele amplicon LBb3 - 05gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 05gq: 5'-ATCATCAAGAGCCTGAACATCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18732837
   
wt amplicon (over InsPos) 18hs - 05gq
wt amplicon start Chr2:18732086
wt amplicon end Chr2:18732837
wt amplicon size 752 bp
Locus specific primer 05gq: 5'-ATCATCAAGAGCCTGAACATCTTT-3'
2nd locus specific primer 18hs: 5'-GTACAACTGGTGAAGGACAACTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11