Genotyping and primer details


Genotyping and primer details for SALK_022689c - At2g40850
Line ID SALK_022689c
Locus At2g40850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17051659 of line SALK_022689c Chr2:17051659
   
Insertion allele amplicon LBb3 - 33vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33vi: 5'-TCACTAGCATATTTCCAGCATGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17052406
   
wt amplicon (over InsPos) 54vw - 33vi
wt amplicon start Chr2:17051037
wt amplicon end Chr2:17052406
wt amplicon size 1370 bp
Locus specific primer 33vi: 5'-TCACTAGCATATTTCCAGCATGTC-3'
2nd locus specific primer 54vw: 5'-GCGGGAATATCAGAAACGTAAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11