Sequences from confirmation amplicon


Confirmation sequences for line SALK_022689c and hit At2g40850 are shown.
Line ID SALK_022689c
Locus At2g40850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17051659
Primers used for PCR 33vi - LBb3, show primer details
   
Sequence Name 42-K117945-SALK_022689C-33vi
GenBank Accession LN485340 [GenBank]
Sequencing primer gene specific primer, 33vi: 5'-TCACTAGCATATTTCCAGCATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117945-SALK_022689C-33vi
TCAGTATTCCATCCTATGAACCGAAGTAGCCGTGAAGCTTCCTGGACCCAATTCTCCTGC
ATCAAAATCATGACCCACAAATCGCTGCAAGGAAGCAACCTTATAGGGCATGGAGGAGAA
AGAGAAGGCATCACTGACATGGAATGGGACATGTGAGATACTAACCAATGCTGTTGGTGG
AACACCAGAAAAGCCTTGGTAATCCAAAAGGTAGGCAGCTAGTTCTCTGATACCCGTCTC
GCCAACAGGAATTGAATGTTTCATTCCCGGTTGCCCGAGCATCAAATTTCCAGACTTTTT
AGGATTATTGAAAGCTAGAGGCTCTTCATCAACCGGTTTAGCCACAGCAATGTTATGTCC
CTTTCCGGTTTGCAAGAGATAAGCACCTCCCATGCCACTTGGTAAAAGCAAAGGCTGAGC
TCCAGAAACCATAGCCATGGTCACCTCAGCCACAAGAGCACGAACGGTGGGCACTCTCTG
TCCACCAAGAATCTCAATCCGTGGAGCCGTGGTTGCTTTGAGATCTTCCTCCATATTAGC
AGACGCAGCTATGGAAAAGCAAGGAGAGGAAACACTACGATGGATGTTGTCATCTTCAAA
CGTATGATTAAAGGATTGCGCGAGGAAGTTGAAGTCAAGGTTGGTAAGGGATTGAAGTCT
GCATCTTTGAGAGGATCGGTTAAAGTAAGGGAACTTGTCCAGGATATCCGCAATGTGTTA
TTAAGTCTCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT
GCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTC
   
Sequence Name 07-K117944-SALK_022689C-R210
GenBank Accession LN484474 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117944-SALK_022689C-R210
GTCATTTGTTACACCACAATATATCCTGGAGACTTAATAACACATTGCGGATATCCTGGA
CAAGTTCCCTTACTTTAACCGATCCTCTCAAAGATGCAGACTTCAATCCCTTACCAACCT
TGACTTCAACTTCCTCGCGCAATCCTTTAATCATACGTTTGAAGATGACAACATCCATCG
TAGTGTTTCCTCTCCTTGCTTTTCCATAGCTGCGTCTGCTAATATGGAGGAAGATCTCAA
AGCAACCACGGCTCCACGGATTGAGATTCTTGGTGGACAGAGAGTGCCCACCGTTCGTGC
TCTTGTGGCTGAGGTGACCATGGCTATGGTTTCTGGAGCTCAGCCTTTGCTTTTACCAAG
TGGCATGGGAGGTGCTTATCTCTTGCAAACCGGAAAGGGACATAACATTGCTGTGGCTAA
ACCGGTTGATGAAGAGCCTCTAGCTTTCAATAATCCTAAAAAGTCTGGAAATTTGATGCT
CGGGCAACCGGGAATGAAACATTCAATTCCTGTTGGCGAGACGGGTATCAGAGAACTAGC
TGCCTACCTTTTGGATTACCAAGGCTTTTCTGGTGTTCCACCAACAGCATTGGTTAGTAT
CTCACATGTCCCATTCCATGTCAGTGATGCCTTCTCTTTCTCCTCCATGCCCTATAAGGT
TGCTTCCTTGCAGCGATTTGTGGGTCATGATTTTGATGCAGGAGAATTGGGTCCAGGAAG
CTTCACGGCTACTTCGGTTCATAGGATTGGAATACTTGATGTGAGACTCCTGAACCTTGA
CAGACATGCTGGAATTTGCCTAGTGA

Last Updated on 06.12.2018 16:11