Genotyping and primer details


Genotyping and primer details for SALK_022993c - At1g74080
Line ID SALK_022993c
Locus At1g74080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27856574 of line SALK_022993c Chr1:27856574
   
Insertion allele amplicon LBb3 - 26mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26mh: 5'-TCAAACATCTTGATGAAAATCTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27857241
   
wt amplicon (over InsPos) 04ng - 26mh
wt amplicon start Chr1:27856248
wt amplicon end Chr1:27857241
wt amplicon size 994 bp
Locus specific primer 26mh: 5'-TCAAACATCTTGATGAAAATCTCG-3'
2nd locus specific primer 04ng: 5'-AGATTGAGATGGGCGAATTACTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11