Genotyping and primer details


Genotyping and primer details for SALK_024560c - At5g06220
Line ID SALK_024560c
Locus At5g06220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1880548 of line SALK_024560c Chr5:1880548
   
Insertion allele amplicon LBb3 - 36wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36wm: 5'-CTTGTACCGTCAAGGTAAACCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1880076
   
wt amplicon (over InsPos) 28xa - 36wm
wt amplicon start Chr5:1880076
wt amplicon end Chr5:1881406
wt amplicon size 1331 bp
Locus specific primer 36wm: 5'-CTTGTACCGTCAAGGTAAACCAAT-3'
2nd locus specific primer 28xa: 5'-AAAACCTCAAAGTCAAACGAGTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11