Genotyping and primer details


Genotyping and primer details for SALK_026656c - At3g22810
Line ID SALK_026656c
Locus At3g22810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8070486 of line SALK_026656c Chr3:8070486
   
Insertion allele amplicon LBb3 - 28eq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28eq: 5'-ATGATGCCAAGAAATGGTTTTAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8070892
   
wt amplicon (over InsPos) 17gc - 28eq
wt amplicon start Chr3:8070120
wt amplicon end Chr3:8070892
wt amplicon size 773 bp
Locus specific primer 28eq: 5'-ATGATGCCAAGAAATGGTTTTAAG-3'
2nd locus specific primer 17gc: 5'-GGAGATATCATGACATTAACCGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11