Genotyping and primer details


Genotyping and primer details for SALK_026688c - At2g39690
Line ID SALK_026688c
Locus At2g39690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16543297 of line SALK_026688c Chr2:16543297
   
Insertion allele amplicon LBb3 - 45et
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45et: 5'-TTTTAAACCTAATGATGCCCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16542562
   
wt amplicon (over InsPos) 05gc - 45et
wt amplicon start Chr2:16542562
wt amplicon end Chr2:16543327
wt amplicon size 766 bp
Locus specific primer 45et: 5'-TTTTAAACCTAATGATGCCCTTTG-3'
2nd locus specific primer 05gc: 5'-ACATCCGAGAGAGAAGTTACCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11