Genotyping and primer details


Genotyping and primer details for SALK_028169c - At3g14020
Line ID SALK_028169c
Locus At3g14020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4643650 of line SALK_028169c Chr3:4643650
   
Insertion allele amplicon LBb3 - BN29
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BN29: 5'-TTTGATTCTTGATGCTTCTTTGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4644081
   
Insertion allele amplicon LBb3 - BN30
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BN30: 5'-AGGTCCACCACTTTTTAACCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4643243
   
wt amplicon (over InsPos) BN30 - BN29
wt amplicon start Chr3:4643243
wt amplicon end Chr3:4644081
wt amplicon size 839 bp
Locus specific primer BN29: 5'-TTTGATTCTTGATGCTTCTTTGTG-3'
2nd locus specific primer BN30: 5'-AGGTCCACCACTTTTTAACCTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11