Genotyping and primer details


Genotyping and primer details for SALK_030147 - At5g18180
Line ID SALK_030147
Locus At5g18180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6008927 of line SALK_030147 Chr5:6008927
   
Insertion allele amplicon R204 - CE89
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CE89: 5'-TCCTGAATAAGAATTGAGCCAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6008285
   
Insertion allele amplicon LBb3 - CE90
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE90: 5'-TGGATTCTTGTGACAGTTTTGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6009293
   
wt amplicon (over InsPos) CE90 - CE89
wt amplicon start Chr5:6008285
wt amplicon end Chr5:6009293
wt amplicon size 1009 bp
Locus specific primer CE89: 5'-TCCTGAATAAGAATTGAGCCAAAC-3'
2nd locus specific primer CE90: 5'-TGGATTCTTGTGACAGTTTTGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11