Sequences from confirmation amplicon


Confirmation sequences for line SALK_030147 and hit At5g18180 are shown.
Line ID SALK_030147
Locus At5g18180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6008927
Primers used for PCR CE89 - R204, show primer details
   
Sequence Name 56-K107203-SALK_030147C-CE89
GenBank Accession LN485637 [GenBank]
Sequencing primer gene specific primer, CE89: 5'-TCCTGAATAAGAATTGAGCCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K107203-SALK_030147C-CE89
CCTCCTACTTGGCTAGAGGTCATCCTGAGTTACAGATATGTGTTTACAGTACTAGAAGAC
GCTTCTGTATGTAATAAAACCTTGGACTCTAATAGTTAATGAGCTTTTTTACTCTCTTGT
TAATAAAACCAAGGTATCAAAAAACTAGAACCAAGAAAACAGTTATCATCAATATGGTTC
TTAAAAAAAGCAATTTAAAGATAGCTTAAGGCTATAGAATATGAATCTAGGCCCTCCCTC
GGCCTCTGAAGCCTCCACGAGGACCACCTCTTGGTTGAAACCCTCTGCTAGCACCTCTGG
GAGCACCTCTACCTCTGAAATTGCCTCTACCACGGGGAGGCCCTCTACCACGGGGAGGGA
CTCTTCCTTCACCACGGCCTCCTGATTGTCCCCTGCAAGAAGAACAGAACAAAGACTTTC
GTTACAGACTTTTTCTAACACTTGAACAAGTTTCTTCTACTACTTCAAGGGACCTAAGAA
TTGGTAACAAGGGTTAAGAAGTGTAGCTTACTTTGGTTGAGGAAGGAATCTTGATAGAGG
TAAAAGCTTCTCAGGGCTAATGAAGAACTTATCGCTGATATATTGTAAACAAATTGACGC
TTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTC
ACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
AACGGGTTCCACG
   
Sequence Name 48-K107202-SALK_030147C-R210
GenBank Accession LN485450 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K107202-SALK_030147C-R210
CGTCATTTGTTACATATATCAGCGATAAGTTCTTCATTAGCCCTGAGAAGCTTTTACCTC
TATCAAGATTCCTTCCTCAACCAAAGTAAGCTACACTTCTTAACCCTTGTTACCAATTCT
TAGGTCCCTTGAAGTAGTAGAAGAAACTTGTTCAAGTGTTAGAAAAAGTCTGTAACGAAA
GTCTTTGTTCTGTTCTTCTTGCAGGGGACAATCAGGAGGCCGTGGTGAAGGAAGAGTCCC
TCCCCGTGGTAGAGGGCCTCCCCGTGGTAGAGGCAATTTCAGAGGTAGAGGTGCTCCCAG
AGGTGCTAGCAGAGGGTTTCAACCAAGAGGTGGTCCTCGTGGAGGCTTCAGAGGCCGAGG
GAGGGCCTAGATTCATATTCTATAGCCTTAAGCTATCTTTAAATTGCTTTTTTTAAGAAC
CATATTGATGATAACTGTTTTCTTGGTTCTAGTTTTTTGATACCTTGGTTTTATTAACAA
GAGAGTAAAAAAGCTCATTAACTATTAGAGTCCAAGGTTTTATTACATACAGAAGCGTCT
TCTAGTACTTGTAAACACATATCTGTTAACTCAGGATGACCTCTAAGCCAAAGTAGGAGG
ATAAAACAAAAAGTAGATTAAAGTTTGGCTCAATTCNNATTCAGGA
Primers used for PCR CE90 - LBb3, show primer details
   
Sequence Name 83-K119135-SALK_030147C-CE90
GenBank Accession LN486263 [GenBank]
Sequencing primer gene specific primer, CE90: 5'-TGGATTCTTGTGACAGTTTTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K119135-SALK_030147C-CE90
TAGAGTTTCTTTCTTGGTGGTGTTATACAGAAGTTGCAACTTTTCTCCATGCTTGTGAGG
GAGATGCTGTGTTCAAGCTCTCCAATGTGAAAATCCCACATTTCAATGCCCCAATCTACC
TACAAAACAAGACCCAGATTGGGAGAGTAGATGAAATCTTTGGTCCTATTAATGAATCTG
TATGTGTTATCTCTCTCTCTCTGTTGTCTTCTTCGTTCACTGGCCTTGCTCACTAATATA
CAAAGCATTGGTTCTTACTTTTTCAGCTATTTTCTATCAAAATGAGGGAAGGTATTGTAG
CATATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 69-K119134-SALK_030147C-R210
GenBank Accession LN485992 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K119134-SALK_030147C-R210
CAATATATATGCTACAATACCTTCCCTCATTTTGATAGAAAATAGCTGAAAAAGTAAGAA
CCAATGCTTTGTATATTAGTGAGCAAGGCCAGTGAACGAAGAAGACAACAGAGAGAGAGA
GATAACACATACAGATTCATTAATAGGACCAAAGATTTCATCTACTCTCCCAATCTGGGT
CTTGTTTTGTAGGTAGATTGGGGCATTGAAATGTGGGATTTTCACATTGGAGAGCTTGAA
CACAGCATCTCCCTCACAAGCATGGAGAAAAGTTGCAACTTCTGTATAACACCACCAAGA
AAGAAACTCTATCATAACAGAGACAATTCACAAGTTTGTTCCTTTTCAATTAACTTCAAA
ACTGTCACAAGAATCC

Last Updated on 06.12.2018 16:11