Genotyping and primer details


Genotyping and primer details for SALK_031878 - At4g25070
Line ID SALK_031878
Locus At4g25070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12876347 of line SALK_031878 Chr4:12876347
   
Insertion allele amplicon R204 - 51aq
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 51aq: 5'-CACAAACTTTCTAGTTTTGCCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12876809
   
wt amplicon (over InsPos) 04cb - 51aq
wt amplicon start Chr4:12875795
wt amplicon end Chr4:12876809
wt amplicon size 1015 bp
Locus specific primer 51aq: 5'-CACAAACTTTCTAGTTTTGCCCTC-3'
2nd locus specific primer 04cb: 5'-TCATCTTAATATCCAGATCCCAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11