Sequences from confirmation amplicon


Confirmation sequences for line SALK_031878 and hit At4g25070 are shown.
Line ID SALK_031878
Locus At4g25070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12876347
Primers used for PCR 51aq - R204, show primer details
   
Sequence Name 04-K107313-SALK_031878C-51aq
GenBank Accession LN484357 [GenBank]
Sequencing primer gene specific primer, 51aq: 5'-CACAAACTTTCTAGTTTTGCCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K107313-SALK_031878C-51aq
CTTTTTGTTCTCAAAAACCTGTATTTGGGTTATTTGGGTGAGGTAACGAAGAGTATTATA
CAAGTAACAACATGGAAGCCAAACATATATCAACAATTGGAACACAGTCTGAAAGCAAAA
CAGATGTAGTATGAATTCTTTTGTGTTTCTTCTAAGCTTTGTCGAGCAGGAAAAAATGAT
TTTGATTACCTTTGTGTGTGTATGTGTATGTTTATGTGAGCGTGTGTCGTGAATAAAGAA
AATTGGAGGCAAAGAGGAGAGTAGAGGTGGTCACTCGTCATTGTAGTCAGAAACGGAATA
AGAAGGAAGAAAAGAGGAGTCGATGAGTTCTTTACGAGATGTTTCCCACAATTGTTGTTC
TATTCCAAGCTTCCTTAGCTCCCTCATTCCTCATAACACATTGTGGTGTAAACAAATTGA
CGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACG
   
Sequence Name 44-K107289-SALK_031878C-R210
GenBank Accession LN485367 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K107289-SALK_031878C-R210
GCGTCATTTGTTACACACAATGTGTTATGAGGAATGAGGGAGCTAAGGAAGCTTGCAATA
GAACAACAATTGTGGGAAACATCTCGTAAAGAACTCATCGACTCCTCTTTTCTTCCTTCT
TATTCCGTTTCTGACTACAATGACGAGTGACCACCTCTACTCTCCTCTTTGCCTCCAATT
TTCTTTATTCACGACACACGCTCACATAAACATACACATACACACACAAAGGTAATCAAA
ATCATTTTTTCCTGCTCGACAAAGCTTAGAAGAAACACAAAAGAATTCATACTACATCTG
TTTTGCTTTCAGACTGTGTTCCAATTGTTGATATATGTTTGGCTTCCATGTTGTTACTTG
TATAATACTCTTCGTTACCTCACCCAAATAACCCAAATACAGGTTTTTTGAGAACAAAAA
GCTGAATTTCATATAGTTAGGGGCAAAAATATAAATTTTATATAGTTGAGGGCAAAACTA
GAAAGTTTGTG

Last Updated on 06.12.2018 16:11