Genotyping and primer details


Genotyping and primer details for SALK_031879 - At5g09600
Line ID SALK_031879
Locus At5g09600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2980305 of line SALK_031879 Chr5:2980305
   
Insertion allele amplicon LBb3 - 39wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39wm: 5'-TAGGTTTGTGTTTGTTTCTTGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15557414
   
wt amplicon (over InsPos) 25xa - 39wm
wt amplicon start Chr5:2981143
wt amplicon end Chr4:15557414
wt amplicon size 1299 bp
Locus specific primer 39wm: 5'-TAGGTTTGTGTTTGTTTCTTGTGC-3'
2nd locus specific primer 25xa: 5'-AAGAAATCAAGAATGCGATCTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11