Genotyping and primer details


Genotyping and primer details for SALK_035775c - At5g48950
Line ID SALK_035775c
Locus At5g48950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19845957 of line SALK_035775c Chr5:19845957
   
Insertion allele amplicon LBb3 - 25md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25md: 5'-GGTGCTTGTTGCTGTAAACCTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19845441
   
Insertion allele amplicon LBb3 - 58xh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 58xh: 5'-TGGTTTAGTCCAATGCATGCTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19847033
   
wt amplicon (over InsPos) 04hb - 36fx
wt amplicon start Chr5:19845172
wt amplicon end Chr5:19846038
wt amplicon size 867 bp
Locus specific primer 36fx: 5'-TGATTTTGAACTAAACAGTCGTGG-3'
2nd locus specific primer 04hb: 5'-GATTTGTGACAATTTTTGGATGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11