Genotyping and primer details


Genotyping and primer details for SALK_036447c - At4g02650
Line ID SALK_036447c
Locus At4g02650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1157221 of line SALK_036447c Chr4:1157221
   
Insertion allele amplicon LBb3 - 30mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30mh: 5'-TCTTGTCTTGAAACCACTTCTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1157815
   
wt amplicon (over InsPos) 01ng - 30mh
wt amplicon start Chr4:1156836
wt amplicon end Chr4:1157815
wt amplicon size 980 bp
Locus specific primer 30mh: 5'-TCTTGTCTTGAAACCACTTCTTCA-3'
2nd locus specific primer 01ng: 5'-ACAGATGGAGATAGAGCCTACGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11