Genotyping and primer details


Genotyping and primer details for SALK_037536 - At5g35750
Line ID SALK_037536
Locus At5g35750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:13913385 of line SALK_037536 Chr5:13913385
   
Insertion allele amplicon R204 - CE97
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CE97: 5'-TCCTGAATAGCTAGATCGAATCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:13912788
   
wt amplicon (over InsPos) CE98 - CE97
wt amplicon start Chr5:13912788
wt amplicon end Chr5:13913650
wt amplicon size 863 bp
Locus specific primer CE97: 5'-TCCTGAATAGCTAGATCGAATCGT-3'
2nd locus specific primer CE98: 5'-GTGCCTTTTGATATGCGTTTTATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11