Genotyping and primer details


Genotyping and primer details for SALK_039445c - At5g20150
Line ID SALK_039445c
Locus At5g20150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6803030 of line SALK_039445c Chr5:6803030
   
Insertion allele amplicon LBb3 - 05cl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 05cl: 5'-GGAATCTTCTTCCATGTCTCATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6803342
   
wt amplicon (over InsPos) 24cu - 05cl
wt amplicon start Chr5:6802614
wt amplicon end Chr5:6803342
wt amplicon size 729 bp
Locus specific primer 05cl: 5'-GGAATCTTCTTCCATGTCTCATCT-3'
2nd locus specific primer 24cu: 5'-TTTCATCCAATTGTTAGAAGACGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11