Genotyping and primer details


Genotyping and primer details for SALK_040299c - At1g03470
Line ID SALK_040299c
Locus At1g03470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:866389 of line SALK_040299c Chr1:866389
   
Insertion allele amplicon LBb3 - 30xl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30xl: 5'-TCTACATCTCACCAGTCTCTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:865751
   
Insertion allele amplicon R204 - BL11
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BL11: 5'-AGCTTTGTTGTTGTTGGAGAAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:867254
   
wt amplicon (over InsPos) BL12 - BL11
wt amplicon start Chr1:866238
wt amplicon end Chr1:867254
wt amplicon size 1017 bp
Locus specific primer BL11: 5'-AGCTTTGTTGTTGTTGGAGAAGAT-3'
2nd locus specific primer BL12: 5'-GGAAGTAGAATCTGTGTTTGGGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11