Genotyping and primer details


Genotyping and primer details for SALK_046323c - At1g80760
Line ID SALK_046323c
Locus At1g80760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30350901 of line SALK_046323c Chr1:30350901
   
Insertion allele amplicon LBb3 - 20ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20ty: 5'-AATGAATTAGAAGCAGCAATCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30350425
   
wt amplicon (over InsPos) 24uu - 20ty
wt amplicon start Chr1:30350425
wt amplicon end Chr1:30351259
wt amplicon size 835 bp
Locus specific primer 20ty: 5'-AATGAATTAGAAGCAGCAATCGTT-3'
2nd locus specific primer 24uu: 5'-TAAATTTATGTTAGGTGCCGGTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11